Transcriptomics

Dataset Information

7

Development of miRNA expression signatures of MEFs deficient in a ER stress mediator ATF6a for tunicamycin treatment


ABSTRACT: To further development of our miRNA expression approach to ER stress, we have employed miRNA microarray expression profiling as a discovery platform to identify ER stress-responsible ones. Mouse embryonic fibroblasts (MEFs) deficient in a ER stress mediator ATF6a were treated with tunicamycin for 12 or 24 hrs. miRNAs responsible for tunicamycin-treatment for 12hrs in ATF6a-dependent manner were extracted. Among them, expression of three miRNAs (miR-26a, miR-27b, miR-143) was quantified in the RNA samples from the same as the microarray by real-time PCR. Mouse embryonic fibroblasts (MEFs) deficient in a ER stress mediator ATF6a were treated with 2ug/mL tunicamycin for 12 or 24 hrs. Two independent experiments were performed at each time (untreated, 12 or 24 hrs). miRNAs responsible for tunicamycin-treatment for 12hrs in ATF6a-dependent manner were extracted.

ORGANISM(S): Mus musculus  

SUBMITTER: Kazue Tsugawa   Naoko Miura  Miho Oyadomari  Mari Funahashi  Tomoko Mori  Kazuna Takahara  Taiji Ito  Kiyoe Kurahashi  Robert Zheng  Ryosuke Sato  Chinobu Miyamoto  Seiichi Oyadomari  Masato Miyake 

PROVIDER: E-GEOD-35176 | ArrayExpress | 2015-01-18

SECONDARY ACCESSION(S): GSE35176PRJNA155987

REPOSITORIES: GEO, ArrayExpress

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