Transcriptomics

Dataset Information

2

GSK3B shRNA in Panc-1 xenografts


ABSTRACT: The Panc-1 human pancreatic cancer line (ATCC) was constructed to stably express control or GSK3B shRNA. Control or GSK3B shRNA expressing Panc-1 cells were implanted subcutaneously into the flanks of 3-5 week old, female, athymic nude mice. Tumors were grown to at least 250mm3. Tumor tissue was microdissected for further analysis. We compared control shRNA (n=4) to GSK3B shRNA (n=3) Panc-1 xenografts. The array data with simple statistical calculations are also provided in a supplementary Excel workbook, with probe-set annotation that we used at the time (users may want newer annotation). We compared xenografts of Panc-1 (Human pancreatic carcinoma cell line, grown in mice) stably expressing GSK3B shRNA (n=3) to similar xenografts with control shRNA (n=4). Xenografts were grown in the flanks of female, athymic nude mice until they reached at least 250mm3. Tumor tissue was microdissected for further analysis. mRNA abundance assays were performed using Affymetrix HG_U133_plus_2 arrays, with 54675 probe-sets.

REANALYSED by: E-GEOD-35351

ORGANISM(S): Homo sapiens  

SUBMITTER: Mahaveer S Bhojani   Rork Kuick  Edgar Ben-Josef  Aaron Spalding  Meredith Morgan 

PROVIDER: E-GEOD-35351 | ArrayExpress | 2014-01-09

SECONDARY ACCESSION(S): GSE35351PRJNA152729

REPOSITORIES: GEO, ArrayExpress

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Publications

Glycogen Synthase Kinase 3β in Pancreatic Cancer and its Implications in Chemotherapy and Radiation Therapy.

Zhang Qiang Q   Bhojani Mahaveer S MS   Ben-Josef Edgar E   Spalding Aaron C AC   Kuick Rork R   Sun Yi Y   Morgan Meredith A MA  

Journal of carcinogenesis & mutagenesis 20130801 3


Pancreatic cancer is a highly lethal disease with a poor prognosis characterized by local and systemic disease progression. Both radiation and chemotherapy play important roles in the management of this disease. However, in order to improve standard therapy many molecularly targeted agents are being developed. Glycogen synthase kinase 3β (GSK3β) participates in a multitude of cellular processes and is a newly proposed therapeutic target in pancreatic cancer. This review will discuss both the onc  ...[more]

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