Transcriptomics

Dataset Information

124

Transcription profiling by array of OsJAZ8deltaC overexpressing rice plants in response to jasmonate


ABSTRACT: The plant hormone jasmonate (JA) plays important roles in the regulation of defense responses in many plants. To clarify the response to JA in rice at gene expression level, we performed a microarray analysis using the Agilent Rice Oligo Microarray (44k, custom-made; Agilent Technologies, Redwood City, CA, USA). As a result, treatment of JA caused high upregulation of many defense-related genes including pathogenesis-related (PR) genes in rice. However, many of these defense-related genes were not upregulated in JA-insensitive transgenic rice plant overexpressing JAZ8deltaC. An Agilent Rice Oligo Microarray (44k, custom-made; Agilent Technologies, Redwood City, CA, USA) was used for the microarray analysis. Total RNA was extracted from leaf blades of four-leaf stage rice plants that had been treated with 100 μM JA for 1 day. For each biological replicate, material from at least three rice plants was pooled to provide a single sample for RNA extraction. We performed 4 biological replicates for each treatment. All microarray procedures and data analyses were performed according to the manufacturer’s instructions. RNA integrity was checked using an Agilent 2100 Bioanalyzer (Agilent Technologies, Redwood City, CA, USA). Total RNA (100 ng) was labeled with Cy-3 using an Agilent Low Input Quick Amp Labeling Kit, One-Color (Agilent Technologies). Fluorescently labeled targets were hybridized to Agilent Rice Oligo Microarrays for 17 h at 65°C. After hybridization, following wash processes were performed according to the manufacturer’s instructions, and hybridized microarrays were scanned using an Agilent Microarray Scanner (G2565CA; Agilent Technologies). Feature extraction software (Agilent Feature Extraction; Agilent Technologies) was used to delineate and measure the signal intensity of each spot in the array, and to normalize intensities. The background was measured around each spot as local background, calculated by the Feature Extraction software. Statistical data extraction processes were performed according to the manufacturer’s instructions.

ORGANISM(S): Oryza sativa Japonica Group  

SUBMITTER: Akihito Kano   Kazuya Akimitsu  Ayumi Miyamoto  kenji gomi  Kenji Gomi  Shoko Yamada 

PROVIDER: E-GEOD-35429 | ArrayExpress | 2012-05-29

SECONDARY ACCESSION(S): GSE35429PRJNA152765

REPOSITORIES: GEO, ArrayExpress

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Publications

Involvement of OsJAZ8 in jasmonate-induced resistance to bacterial blight in rice.

Yamada Shoko S   Kano Akihito A   Tamaoki Daisuke D   Miyamoto Ayumi A   Shishido Hodaka H   Miyoshi Seika S   Taniguchi Shiduku S   Akimitsu Kazuya K   Gomi Kenji K  

Plant and Cell Physiology 20121026 12


The plant hormone jasmonic acid (JA) has a crucial role in both host immunity and development in plants. Here, we report the importance of JA signaling in the defense system of rice. Exogenous application of JA conferred resistance to bacterial blight caused by Xanthomonas oryzae pv. oryzae (Xoo) in rice. Expression of OsJAZ8, a rice jasmonate ZIM-domain protein, was highly up-regulated by JA. OsJAZ8 interacted with a putative OsCOI1, which is a component of the SCF(COI1) E3 ubiquitin ligase com  ...[more]

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