Transcriptomics

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CSB3 transcriptome analysis (Arabidopsis thaliana)


ABSTRACT: In this study we show the comparative transcriptome of constitutive subtilisin3 (csb3) plants, an Arabidopsis mutant showing strikingly enhanced resistance to biotrophic pathogens. CSB3 encodes a 1-hydroxy-2-methyl-2-butenyl 4-diphosphate synthase, the enzyme controlling the penultimate step of the biosynthesis of isopentenyl diphosphate via the 2-C-methyl-d-erythritol-4-phosphate (MEP) pathway in the chloroplast. It has been proposed that CSB3 represents a point of metabolic convergence modulating the magnitude of SA-mediated disease resistance to biotrophic pathogens. We show that csb3 have increased expression of a set of genes encoding defense-related proteins and enzymes, which includes two subtilisins. In essence our results substantiates an important role of these two subtilases in the activation of defense-related signaling pathways against biotrophic pathogens. 5 five-week-old plants were collected, frozen in liquid nitrogen and stored at -80ºC. Samples from Col-0 and csb3 were compared.

ORGANISM(S): Arabidopsis thaliana  

SUBMITTER: Pablo Vera   Vicente Ramírez  María J Gil  Ana López 

PROVIDER: E-GEOD-35507 | ArrayExpress | 2014-04-25

SECONDARY ACCESSION(S): GSE35507PRJNA152315

REPOSITORIES: GEO, ArrayExpress

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Publications

An extracellular subtilase switch for immune priming in Arabidopsis.

Ramírez Vicente V   López Ana A   Mauch-Mani Brigitte B   Gil Ma José MJ   Vera Pablo P  

PLoS pathogens 20130620 6


In higher eukaryotes, induced resistance associates with acquisition of a priming state of the cells for a more effective activation of innate immunity; however, the nature of the components for mounting this type of immunological memory is not well known. We identified an extracellular subtilase from Arabidopsis, SBT3.3, the overexpression of which enhances innate immune responses while the loss of function compromises them. SBT3.3 expression initiates a durable autoinduction mechanism that pro  ...[more]

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