Transcriptomics

Dataset Information

5

Pulmonary gene expression in MutaTMMouse orally gavaged with three doses of benzo(a)pyrene and vehicle control


ABSTRACT: In this study, we investigate mRNA profiles in lung of mice exposed to benzo(a)pyrene. Male mice were exposed to three doses (25, 50, and 75 mg/kg/day BaP) for 28 days and profiles were examined three days post-exposure. Our analyses reveal that BaP causes pulmonary specific cellular transformation indicative of carcinogenesis. Individual total RNA (200 ng) from 5 mice per treatment group (control, 25mg/kg/day, 50mg/kg/day, and 75mg/kg/day) and universal reference total RNA (Stratagene, Mississauga, ON, Canada) was used to synthesize double stranded cDNA.

ORGANISM(S): Mus musculus  

SUBMITTER: Sabina Halappanavar   Sarah Labib  Andrew Williams  Paul A White  Volker Arlt 

PROVIDER: E-GEOD-35718 | ArrayExpress | 2012-10-03

SECONDARY ACCESSION(S): GSE35718PRJNA152117

REPOSITORIES: GEO, ArrayExpress

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Publications

Subchronic oral exposure to benzo(a)pyrene leads to distinct transcriptomic changes in the lungs that are related to carcinogenesis.

Labib Sarah S   Yauk Carole C   Yauk Carole C   Williams Andrew A   Arlt Volker M VM   Phillips David H DH   White Paul A PA   Halappanavar Sabina S  

Toxicological sciences : an official journal of the Society of Toxicology 20120518 1


We have previously shown that acute oral exposure to the environmental carcinogen benzo(a)pyrene (BaP) elicits comparable levels of DNA adducts, but distinct transcriptomic changes, in mouse lungs and livers, the two main BaP bioactivating organs. Oral BaP exposure is predominantly associated with lung cancer and not hepatic cancer in some animal models, suggesting that gene expression differences may provide insight into the drivers of tissue-specific carcinogenesis. In the present study, we ex  ...[more]

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