Transcriptomics

Dataset Information

4

Increased mutability of staphylococci in biofilms as a consequence of oxidative stress


ABSTRACT: In this work we have demonstrated increased mutability of Staphylococcus aureus and S. epidermidis in biofilms and have explored the mechanisms underlying the enhanced mutability. A novel static biofilm model, utilising cellulose filter disks, was developed to support the formation of mature biofilms with sufficiently high cell densities to permit determination of mutation frequencies. The mutability of biofilm cultures increased up to 60 fold and 4 fold for S. aureus and S. epidermidis, respectively, compared with planktonic cultures. Incorporation of antioxidants into S. aureus biofilms reduced mutation frequencies, indicating that increased oxidative stress underlies increased mutability in the biofilm. Transcriptional profiling revealed upregulation of the superoxide dismutase gene, sodA, in early biofilm cultures, also suggesting enhanced oxidative stress in these cultures. However, loss of the genes encoding superoxide dismutases or peroxidases did not specifically exacerabate biofilm mutability. In S. aureus SH1000, hydrogen peroxide was found to contribute to biofilm mutability. Three growth conditions (18 hr planktonic growth, 48 hr biofilm growth and 144 biofilm growth) of which there are three biological replicates of each

ORGANISM(S): Staphylococcus aureus  

SUBMITTER: Alex J O'Neill   Ian Chopra  Victoria Ryder  Victoria J Ryder 

PROVIDER: E-GEOD-35837 | ArrayExpress | 2012-03-01

SECONDARY ACCESSION(S): GSE35837PRJNA151965

REPOSITORIES: GEO, ArrayExpress

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