Transcriptomics

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Transcription profiling by array of rice dwarf d10-1 and d14-1 mutants treated with GR24, a synthetic strigolactone analog


ABSTRACT: Strigolactones have been defined as a new group of phytohormones that regulate shoot branching. The phenotypes of strigolacton-related rice (Oryza sativa) dwarf (d) mutants demonstrated that SLs inhibit mesocotyl elongation by controlling cell division. Moreover, the trans-zeatin, one of active cytokinins, content of mesocotyls was increased in the SL-deficient d10-1 and SL-insensitive d14-1 mutants. To examine if there are genes related to cytokinin-biosynthesis or -degradation among the strigolactone-responsive genes expressed in the mesocotyl, we carried out microarray analyses using d10-1, d14-1 and the wild-type grown under dark conditions, with or without pretreatment of a synthetic strigolactone analog, GR24. Gene expression profiles in 4-day-old mesocotyls of dwarf (d) mutants (d10-1 and d14-1) and wild-type germinated under dark conditions with or without treatment of 1 μM GR24 were analyzed. Three biological replicates were prepared for each conditions, and a total of eighteen samples were analyzed.

REANALYSED by: E-GEOD-36271

ORGANISM(S): Oryza sativa Japonica Group  

SUBMITTER: Jinghua Yang  Tomoko Tsuchida-Mayama   Junko Kyozuka   Hiroaki Ichikawa   Yusuke Jikumaru   Takaki Yamauchi   Nobuhiro Tsutsumi   Shinjiro Yamaguchi   Yoshiaki Nagamura   Zhongyuan Hu   Itsuro Takamure   Mikio Nakazono    

PROVIDER: E-GEOD-36271 | ArrayExpress | 2013-12-31

SECONDARY ACCESSION(S): GSE36271PRJNA153103

REPOSITORIES: GEO, ArrayExpress

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