Dataset Information


Induction of lignocellulose-degrading enzymes in Neurospora crassa by cellodextrins

ABSTRACT: Transcriptional profiling with next-generation sequencing methods demonstrated that a Neurospora crassa mutant with the three most highly expressed beta-glucosidase genes deleted had a transcriptional response to cellobiose similair to that of wild type N. crassa exposed to cellulose. N. crassa was pregrown in Sucrose and transferred to Avicel (cellulose), Cellobiose, Sucrose or media with no carbon added. Biological triplicates used to identify differentially expressed genes in WT on Avicel. Single libraries for mutant strains identify which genes show similair expression on cellobiose as in the WT on cellulose.

ORGANISM(S): Neurospora crassa OR74A  

SUBMITTER: Elizabeth A Znameroski   Jamie H Cate  Samuel T Coradetti  N L Glass  Samuel Coradetti 

PROVIDER: E-GEOD-36719 | ArrayExpress | 2012-04-04



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Induction of lignocellulose-degrading enzymes in Neurospora crassa by cellodextrins.

Znameroski Elizabeth A EA   Coradetti Samuel T ST   Roche Christine M CM   Tsai Jordan C JC   Iavarone Anthony T AT   Cate Jamie H D JH   Glass N Louise NL  

Proceedings of the National Academy of Sciences of the United States of America 20120402 16

Neurospora crassa colonizes burnt grasslands in the wild and metabolizes both cellulose and hemicellulose from plant cell walls. When switched from a favored carbon source such as sucrose to cellulose, N. crassa dramatically upregulates expression and secretion of a wide variety of genes encoding lignocellulolytic enzymes. However, the means by which N. crassa and other filamentous fungi sense the presence of cellulose in the environment remains unclear. Here, we show that an N. crassa mutant ca  ...[more]

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