Dataset Information


A pioneer protein encoded by required to maintain repression2 facilitates locus-specific paramutation in Zea mays

ABSTRACT: This research identifies a novel protein required for paramutation at the maize purple plant1 locus. This 'required to maintain repression2' (RMR2) protein represents the founding member of a plant-specific clade of hypothetical proteins. We show that RMR2 is required for transcriptional repression at the Pl1-Rhoades haplotype, for accumulation of 24 nt RNA species, and for maintenance of a 5-methylcytosine pattern distinct from that maintained by RNA polymerase IV. Genetic tests indicate that RMR2 is not required for paramutation occurring at the red1 locus. These results distinguish the paramutation-type mechanisms operating at specific haplotypes. The RMR2 clade of proteins provides a new entry point for understanding the diversity of epigenomic control operating in higher plants. Examination of small RNAs using Illumina's sequencing-by-synthesis (SBS) platform to deep sequence small RNA libraries made from the 4-cm cobs of rmr2 mutant and non-mutant siblings.

ORGANISM(S): Zea mays  

SUBMITTER: Jay B Hollick   Rosalind Williams-Carrier  Jerry Kermicle  Jennifer L Stonaker  Alice Barkane  Blake C. Meyers  Jana P Lim  Blake C Meyers  Clarissa C Lee  Irene T Liao  Joy-El Barboura  Stacey A Simon  Susan E Parkinson 

PROVIDER: E-GEOD-37204 | ArrayExpress | 2012-04-16



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Meiotically heritable epigenetic changes in gene regulation known as paramutations are facilitated by poorly understood trans-homolog interactions. Mutations affecting paramutations in maize (Zea mays) identify components required for the accumulation of 24-nucleotide RNAs. Some of these components have Arabidopsis thaliana orthologs that are part of an RNA-directed DNA methylation (RdDM) pathway. It remains unclear if small RNAs actually mediate paramutations and whether the maize-specific mole  ...[more]

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