Transcriptomics

Dataset Information

4

Effect of obacunone on Salmonella Typhimurium transcriptome


ABSTRACT: Obacunone is a limonoids present in Citrus species. We previously reported that obacunone was inhibitory to the cell-cell signaling in Vibrio harveyi and Escherichia coli O157:H7. In the present work we evaluated the effect of obacunone on the food borne pathogen Salmonella Typhimurium LT2 using cDNA microarray. The results demonstrate that obacunone exerts an antivirulence effect on S. Typhimurium LT2 by repressing SPI1 and SPI2. Furthermore, the effect of obacunone seems to be dependent upon EnvZ. One condition experiment, obacunone treated versus DMSO treated. Biological replicates: 3 control, 3 treatment, hybridized in dye-swapped design

ORGANISM(S): Salmonella enterica subsp. enterica serovar Typhimurium str. LT2  

SUBMITTER: G K Jayaprakasha   Bhimanagouda S Patil  Palmy R Jesudhasan  Suresh D Pillai  Amit Vikram 

PROVIDER: E-GEOD-37234 | ArrayExpress | 2012-08-21

SECONDARY ACCESSION(S): GSE37234PRJNA158961

REPOSITORIES: GEO, ArrayExpress

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Publications

Obacunone represses Salmonella pathogenicity islands 1 and 2 in an envZ-dependent fashion.

Vikram Amit A   Jayaprakasha Guddadarangavvanahally K GK   Jesudhasan Palmy R PR   Pillai Suresh D SD   Patil Bhimanagouda S BS  

Applied and environmental microbiology 20120727 19


Obacunone belongs to a class of unique triterpenoids called limonoids, present in Citrus species. Previous studies from our laboratory suggested that obacunone possesses antivirulence activity and demonstrates inhibition of cell-cell signaling in Vibrio harveyi and Escherichia coli O157:H7. The present work sought to determine the effect of obacunone on the food-borne pathogen Salmonella enterica serovar Typhimurium LT2 by using a cDNA microarray. Transcriptomic studies indicated that obacunone  ...[more]

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