Transcriptomics

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Transcript profiling of different Arabidopsis thaliana ecotypes in response to Tobacco etch potyvirus infection


ABSTRACT: We have used a strain of Tobacco etch potyvirus (TEV) experimentally adapted to Arabidopsis thaliana ecotype Ler-0 to infect a set of seven A. thaliana plant ecotypes(Col-0, Ei-2, Wt-1, ler-0, Oy-0, St-0). Each ecotype was inoculated with the same amount of the virus. Using commercial microarrays containing probes Arabidopsis thaliana ssp. Col-0 plant transcripts, we explored the effect of viral infection in the plant transcriptome This microarray can be useful to study gene activity of Arabidopsis thaliana associated with response to virus infection. For ecotypes ST-0, Wt-1, Ler-0, Di-2 there are five biological replicates and for ecotype Oy-0 there are three. As control we used four technical replicates of each ecotype, but five for Ler-0. Rows and columns are numbered as scanned by a GenePix Scanner (barcode on the bottom, DNA on the front surface).

ORGANISM(S): Arabidopsis thaliana  

SUBMITTER: Santiago F Elena   José M Cuevas  Julia Hillung 

PROVIDER: E-GEOD-37269 | ArrayExpress | 2012-07-03

SECONDARY ACCESSION(S): GSE37269PRJNA159163

REPOSITORIES: GEO, ArrayExpress

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Transcript Profiling of Different Arabidopsis thaliana Ecotypes in Response to Tobacco etch potyvirus Infection.

Hillung Julia J   Cuevas José M JM   Elena Santiago F SF  

Frontiers in microbiology 20120625


The use of high-throughput transcript profiling techniques has opened the possibility of identifying, in a single experiment, multiple host mRNAs whose levels of accumulation are altered in response to virus infection. Several studies have used this approach to analyze the response of Arabidopsis thaliana to the infection by different RNA and DNA viruses. However, the possible differences in response of genetically heterogeneous ecotypes of the plant to the same virus have never been addressed b  ...[more]

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