Dataset Information


Gene expression profiling of rhesus macaques vaccinated with ALVAC-SIVgpe DNA + SIVgp120 protein subunit and unvaccinated controls after challenge with SIVmac251 - 3 wks post-infection

ABSTRACT: The SIVmac251 macaque model has been used to evaluate the efficacy of vaccine for HIV. Exposure of macaques to a single high dose of SIVmac251 results in transmission of multiple viral variants, which contrasts the few HIV variants typically transmitted in humans. In here, we investigated whether the dose of SIVmac251 challenge affected vaccination efficacy and found that exposure of the immunized macaques to single high dose of SIVmac251 resulted in no vaccine efficacy, whereas exposure to a tenfold lower dose resulted in protection from SIVmac251 acquisition and protection from disease in animals that become infected. The dose of challenge did not affect the expression of inflammatory genes in the gut in acute infection, but at set point, a significant down regulation of interferon responsive genes and up regulation of genes involved in B and T-cell responses, was observed only in vaccinated animals exposed to a lower dose of SIVmac251. Accordingly, in these animals, we also found a significant correlation with vaccine induced T-cell responses and protection from disease. These data demonstrate that the evaluation of the efficacy of vaccine candidates for HIV relies on accurate modeling in macaques to better mimic HIV transmission to humans. A total of 31 RNA samples were hybridized on to Rhesus Affymetrix 3' Expression arrays. The study was composed of 8 vaccinated and 10 control animals subjected to a low dose challenge and 6 vaccinated and 7 control animals subjected to a high dose challenge

ORGANISM(S): Macaca mulatta  

SUBMITTER: Anna Hryniewicz   David Venzon  Tia Morgan  David Montefiori  Chris Miller  Monica Vaccari  Genoveffa Franchini  Claudio Fenizia  Steven E Bosinger  Youngjun Guan  Donald Forthal  James Tartaglia  Barbara Felber  Melvin Doster  Justin Pollara  Zhong-Min Ma  Brandon F Keele  Gregory K Tharp  George Pavlakis  Guido Silvestri  Jeffrey D Lifson  Guido Ferrari 

PROVIDER: E-GEOD-37311 | ArrayExpress | 2013-03-01



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