Transcriptomics

Dataset Information

476

Dicer Partner Proteins Tune the Length of Mature miRNAs in Flies and Mammals


ABSTRACT: Drosophila Dicer-1 produces microRNAs (miRNAs) from pre-miRNA, whereas Dicer-2 generates small interfering RNAs (siRNAs) from long dsRNA. loquacious (loqs) encodes three Dicer partner proteins, Loqs-PA, Loqs-PB, and, Loqs-PD, generated by alternative splicing. To understand the function of each Loqs isoform, we constructed loqs isoform-specific rescue flies. Loqs-PD promotes siRNA production in vivo by Dicer-2. Loqs-PA or Loqs-PB is required for viability, but the proteins are not fully redundant: Loqs-PB is required to produce a specific subset of miRNAs. Surprisingly, Loqs-PB tunes the product size cleaved by Dicer-1 from pre-miR-307a, generating a longer miRNA isoform with a distinct seed sequence and target specificity. The mouse and human Dicer-binding partner TRBP, a homolog of Loqs-PB, similarly tunes the site of pre-miR-132 cleavage by mammalian Dicer. Thus, Dicer-binding partner proteins can change the choice of cleavage site by Dicer, producing miRNAs with different target specificities than those that would be made by Dicer alone. Examination of Dicer-binding proteins on small RNA profiles of female fly heads, fly ovaries, mouse embryonic fibroblasts, and mouse tail fibroblasts.

ORGANISM(S): Musculus  

SUBMITTER: Jia Xu   Jui H Hung  Ryuya Fukunaga  Phillip D Zamore  Bo W Han  Zhiping Weng 

PROVIDER: E-GEOD-37443 | ArrayExpress | 2012-12-07

SECONDARY ACCESSION(S): SRP012343GSE37443PRJNA159933

REPOSITORIES: GEO, ArrayExpress, ENA

altmetric image

Publications

Dicer partner proteins tune the length of mature miRNAs in flies and mammals.

Fukunaga Ryuya R   Han Bo W BW   Hung Jui-Hung JH   Xu Jia J   Weng Zhiping Z   Zamore Phillip D PD  

Cell 20121011 3


Drosophila Dicer-1 produces microRNAs (miRNAs) from pre-miRNA, whereas Dicer-2 generates small interfering RNAs (siRNAs) from long dsRNA. Alternative splicing of the loquacious (loqs) mRNA generates three distinct Dicer partner proteins. To understand the function of each, we constructed flies expressing Loqs-PA, Loqs-PB, or Loqs-PD. Loqs-PD promotes both endo- and exo-siRNA production by Dicer-2. Loqs-PA or Loqs-PB is required for viability, but the proteins are not fully redundant: a specific  ...[more]

Similar Datasets

2014-04-21 | E-GEOD-38636 | ArrayExpress
2012-11-28 | E-GEOD-35638 | ArrayExpress
2010-02-23 | E-GEOD-19734 | ArrayExpress
2011-01-14 | E-GEOD-25897 | ArrayExpress
2010-04-28 | E-GEOD-20592 | ArrayExpress
2013-06-05 | E-GEOD-47650 | ArrayExpress
2012-10-01 | E-GEOD-37696 | ArrayExpress
2013-07-01 | E-GEOD-44062 | ArrayExpress
2013-04-03 | E-GEOD-39275 | ArrayExpress
2014-05-04 | E-GEOD-47462 | ArrayExpress