Dataset Information


Expression profiling in young spikes of pistillody wheat line compared with normal wheat line

ABSTRACT: We have employed whole genome microarray expression profiling to identify genes conferring induction of pistillody, homeotic transformation of stamens into pistil-like structures. As a result, we identified five genes which show higher expression levels in pistillody line compared with normal line. Quantitative expression analysis using real-time PCR indicated that among five genes a calmodulin (CaM)-binding protein gene, WCBP1 (wheat calmodulin-binding protein 1), is obviously up-regulated in the young spikes of the pistillody line. The full-length cDNA sequence for WCBP1 showed it is a member of the ACBP60 family CaM-binding protein. Expression patterns were compared between the pistillody line and normal line. Total RNA samples were isolated from young spikes (3-10mm in length) at floret differentiation stage. Two independent experiments were conducted in each experiments.

ORGANISM(S): Triticum aestivum  

SUBMITTER: Yasunari Ogihara   Kanako Kawaura  Koji Murai 

PROVIDER: E-GEOD-37486 | ArrayExpress | 2012-12-31



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