Transcriptomics

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Identification of novel lung genes in bronchial epithelium by serial analysis of gene expression


ABSTRACT: A description of the transcriptome of human bronchial epithelium should provide a basis for studying lung diseases including cancer. We demonstrate here that minute epithelial specimens obtained by bronchial brushings afford reliable profiling by serial analysis of gene expression (SAGE) leading to lung gene discovery. We have deduced global gene expression profiles of bronchial epithelium and lung parenchyma, based upon a vast data set of nearly two million sequence tags from 21 SAGE libraries generated from individuals with a history of smoking. Cluster and linear regression analysis demonstrate the repeatability and reproducibility of bronchial SAGE libraries, and suggest that the transcriptome of the bronchial epithelium is distinct from that of lung parenchyma and other tissue types. This distinction is highlighted by the abundant expression of signature genes that reflect tissue-specific and region-specific functions. Through our analysis we have identified novel bronchial-enriched genes and a novel transcript variant for surfactant, pulmonary-associated protein B in lung parenchyma. Conspicuously, gene expression associated with ciliogenesis is evident in bronchial epithelium. Additionally, it is noted that a large number of unmapped tags awaits further investigation. This study represents a comprehensive delineation of the bronchial and parenchyma transcriptomes, identifying more than 20,000 known and hypothetical genes expressed in the human lung, constituting one of the largest human SAGE studies reported to date. Keywords: SuperSeries This reference Series links data in the following related Series: GSE3707 Expression profiling of bronchial epithelium GSE3708 Expression profiling of normal lung parenchyma

ORGANISM(S): Homo sapiens  

SUBMITTER: Raj Chari   Ming-Sound Tsao  Ronald J deLeeuw  Wan L Lam  Stephen Lam  Marco Marra  Ashleen Shadeo  Calum MacAulay  Steven Jones  Kim Marie Lonergan  Raymond Ng  Victor Ling  Bryan Chi  Kim M Lonergan 

PROVIDER: E-GEOD-3754 | ArrayExpress | 2006-11-20

SECONDARY ACCESSION(S): GSE3754PRJNA93925

REPOSITORIES: GEO, ArrayExpress

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