Transcriptomics

Dataset Information

2

The global gene expression pattern between human fibroblasts and human neural stem cells


ABSTRACT: Recent advances in direct reprogramming using cell type-specific transcription factors provide an unprecedented opportunity for rapid generation of desired human cell types from easily accessible tissues. However, due to the diversity of conversion factors that facilitate the process, an arduous screening step is inevitable to find the appropriate combination(s). Here, we show that under chemically defined conditions minimal pluripotency factors are sufficient to directly reprogram human fibroblasts into stably self-renewing neural progenitor/stem cells (NSCs), but without passing through a pluripotent intermediate stage. These NSCs can be expanded and propagated in vitro without losing their potential to differentiate into various neuronal subtypes and glia. Our direct reprogramming strategy represents a simple and advanced paradigm of direct conversion that will provide an unlimited source of human neural cells for cell therapy, disease modeling, and drug screening. We used microarray to compare the global gene expression pattern between human fibroblasts and human neural epitheliums from human ESCs or directly from fibroblasts. We cultured cells and harvested them and then extracted total RNA for microarray.

ORGANISM(S): Homo sapiens  

SUBMITTER: Sheng Ding   Janghwan Kim  Saiyong Zhu 

PROVIDER: E-GEOD-38045 | ArrayExpress | 2013-01-16

SECONDARY ACCESSION(S): GSE38045PRJNA167127

REPOSITORIES: GEO, ArrayExpress

Dataset's files

Source:
Action DRS
E-GEOD-38045.idf.txt Idf
E-GEOD-38045.processed.1.zip Processed
E-GEOD-38045.raw.1.zip Raw
E-GEOD-38045.sdrf.txt Txt
Items per page:
1 - 4 of 4

Similar Datasets

1000-01-01 | S-EPMC4834053 | BioStudies
2017-01-01 | GSE64882 | GEO
2015-01-01 | S-EPMC4397673 | BioStudies
1000-01-01 | S-EPMC6158546 | BioStudies
1000-01-01 | S-EPMC4120813 | BioStudies
1000-01-01 | S-EPMC4297873 | BioStudies
2013-01-01 | S-EPMC3637783 | BioStudies
2012-01-01 | S-EPMC3399516 | BioStudies
2011-01-01 | S-EPMC3123318 | BioStudies
2012-04-07 | E-GEOD-36246 | BioStudies