Dataset Information


Gene expression in mouse pre-B cells transduced with Ikaros.

ABSTRACT: Ikaros family DNA binding proteins are critical regulators of B cell development. To identify Ikaros-regulated genes in pre-B cells we performed gene expression studies at enhanced temporal resolution. We used retroviral gene transfer to express Ikaros proteins in the pre-B cell line B3. Total RNA from 2 biological replicates of B3 cells transduced with wild type Ikaros (HA-Ikaros-IRES-GFP) and DNA binding-deficient Ikaros mutant 159A (HA-159A Ikaros-IRES-GFP) was isolated 48h after infection. To increase the temporal resolution of Ikaros-regulated gene expression we transduced B3 cells with inducible Ikaros (HA-Ikaros-ERt2-IRES-GFP) and isolated total RNA from 3 biological replicates after 2 h and 6 h of exposure to 4-hydroxytamoxifen. Vector transduced B3 cells (ERt2-IRES-GFP) treated with 4-hydroxytamoxifen were used as controls.

ORGANISM(S): Mus musculus  

SUBMITTER: Isabel F Vidal   Matthias Merkenschlager  Tom Carroll  Gopuraja Dharmalingam 

PROVIDER: E-GEOD-38110 | ArrayExpress | 2013-01-15



altmetric image


Ikaros family DNA-binding proteins are critical regulators of B-cell development. Because the current knowledge of Ikaros targets in B-cell progenitors is limited, we have identified genes that are bound and regulated by Ikaros in pre-B cells. To elucidate the role of Ikaros in B-cell lineage specification and differentiation, we analyzed the differential expression of Ikaros targets during the progression of multipotent to lymphoid-restricted progenitors, B- and T-cell lineage specification, an  ...[more]

Similar Datasets

2013-01-15 | E-GEOD-38169 | ArrayExpress
2013-01-15 | E-GEOD-42551 | ArrayExpress
2013-01-15 | E-GEOD-38200 | ArrayExpress
2019-03-05 | PXD003263 | Pride
2015-11-03 | E-GEOD-72801 | ArrayExpress
2013-01-15 | E-GEOD-42462 | ArrayExpress
2015-02-02 | E-GEOD-64899 | ArrayExpress
2015-02-02 | E-GEOD-64893 | ArrayExpress
2015-02-02 | E-GEOD-54447 | ArrayExpress
2012-09-14 | E-GEOD-27938 | ArrayExpress