Transcriptomics

Dataset Information

3

Molecular analysis of growth-limiting drought stress in Brachypodium leaves


ABSTRACT: To explore the usefulness of Brachypodium distachyon for drought studies, a reproducible in soil drought assay was developed. Spontaneous soil drying led to a 45% reduction in leaf size, and this most mostly due to a decrease in cell expansion, whereas cell division remained largely unaffected by drought. To investigate the molecular basis of the observed leaf growth reduction, Brachypodium leaf 3 was dissected in three zones, namely the proliferation, expansion and mature zone, and subjected to transcriptome analysis using a Affymetrix whole-genome tiling array. This approach allowed us to highlight that transcriptome profiles of different developmental leaf zones respond differently to drought. Several genes and biological processes involved in drought tolerance were identified. Mainly, we observed an increased energy availability in the proliferation zone along with an upregulation of sterol synthesis that may influence membrane fluidity. 24 samples of Brachypodium leaf 3 harvested about 24 hours after the emergence of leaf 3, mild drought stress was performed for 2 biological replicates, severe drought stress and control condition was performed for 3 biological replicates. 3 developmental zones, namely the proliferation, expansion and mature zone, are dissected from leaf 3.

ORGANISM(S): Brachypodium distachyon  

SUBMITTER: Dirk Inzé   Wim Verelst  Stefanie De Bodt 

PROVIDER: E-GEOD-38247 | ArrayExpress | 2012-10-01

SECONDARY ACCESSION(S): GSE38247PRJNA167521

REPOSITORIES: GEO, ArrayExpress

Similar Datasets

2015-08-28 | E-GEOD-55592 | ArrayExpress
2019-03-26 | PXD008977 | Pride
2020-06-04 | PXD017441 | Pride
2017-12-25 | E-MTAB-4994 | ArrayExpress
2016-11-08 | PXD005238 | Pride
2012-09-26 | E-GEOD-41159 | ArrayExpress
2015-09-03 | E-GEOD-67551 | ArrayExpress
2016-07-06 | E-GEOD-77826 | ArrayExpress
2018-01-31 | PXD004118 | Pride
2016-09-15 | PXD004096 | Pride