Transcriptomics

Dataset Information

53

Identification of potential miRNAs and their targets in Vriesea carinata (Poales, Bromeliaceae) [small RNA-seq]


ABSTRACT: miRNAs are small non-coding regulatory RNAs that play important functions in the regulation of gene expression at the post-transcriptional level by targeting mRNAs for degradation or by inhibiting protein translation. Bromeliaceae family is an example of a large and well described adaptive radiation of plant families in the Neotropics. This family is composed of terrestrial xerophytes and both facultative and obligatory epiphytes, occurring in a wide range of habitats. Bromeliads have different habits, varying from terrestrial to epiphytical, and are found from sea level to altitudes above 4,000 m, in both desert and humid regions, as well as in soils subject to regular floods and in places with very little or great luminosity. This huge habitat plasticity makes bromeliads an interesting model to study the expression of miRNAs in different natural conditions and the first step is to identify miRNAs and its targets. For this purpose, we used a high-throughput sequencing analysis (Solexa technology) of small RNAs (sRNAs) from the endemic South American species Vriesea carinata. microRNA profiles in 1 leaf library of Vriesea carinata by deep sequencing (Illumina HiSeq2000)

ORGANISM(S): Vriesea carinata  

SUBMITTER: Frank F Guzman  Rogerio Margis   Rogério R Margis   Mauricio M Almerao    

PROVIDER: E-GEOD-38249 | ArrayExpress | 2014-06-02

SECONDARY ACCESSION(S): GSE38249PRJNA167589

REPOSITORIES: GEO, ArrayExpress

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Publications

Identification of potential miRNAs and their targets in Vriesea carinata (Poales, Bromeliaceae).

Guzman Frank F   Almerão Mauricio Pereira MP   Korbes Ana Paula AP   Christoff Ana Paula AP   Zanella Camila Martini CM   Bered Fernanda F   Margis Rogério R  

Plant science : an international journal of experimental plant biology 20130602


The miRNAs play important roles in regulation of gene expression at the post-transcriptional level. A small RNA and RNA-seq of libraries were constructed to identify miRNAs in Vriesea carinata, a native bromeliad species from Brazilian Atlantic Rainforest. Illumina technology was used to perform high throughput sequencing and data was analyzed using bioinformatics tools. We obtained 2,191,509 mature miRNAs sequences representing 54 conserved families in plant species. Further analysis allowed th  ...[more]

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