Genomics

Dataset Information

297

RNA polymerase II collision interrupts convergent transcription (ChIP-seq)


ABSTRACT: Anti-sense non-coding transcripts, genes-within-genes, and convergent gene pairs are prevalent among eukaryotes. The existence of such transcription units raises the question of what happens when RNA polymerase II (RNAPII) molecules collide head-to-head. Here we use a combination of biochemical and genetic approaches in yeast to show that polymerases transcribing opposite DNA strands cannot bypass each other. RNAPII stops, but does not dissociate upon head-to-head collision in vitro, suggesting that opposing polymerases represent insurmountable obstacles for each other. Head-to-head collision in vivo results in RNAPII stopping as well, and removal of collided RNAPII from the DNA template can be achieved via ubiquitylation-directed proteolysis. Indeed, in cells lacking efficient RNAPII poly-ubiquitylation, the half-life of collided polymerases increases, so that these can be detected between convergent genes by ChIP-Seq. These results provide new insight into fundamental mechanisms of gene traffic control, and point to an unexplored effect of anti-sense transcription on gene regulation via polymerase collision. ChIP-Seq of RNA polymerase II was performed with WT and Elongain C deletion mutant (elc1∆) cells. 4H8 antibody against the Rpb1 C-terminal domain was used for RNA polymerase II immunoprecipitation, whilst mouse IgG antibody was used for control immunoprecipitations. Two biological replicates were performed for both WT and elc1∆.

ORGANISM(S): Saccharomyces cerevisiae  

SUBMITTER: Jesper Q Svejstrup   Lars Steinmetz  David J Hobson  Wu Wei 

PROVIDER: E-GEOD-38382 | ArrayExpress | 2012-11-30

SECONDARY ACCESSION(S): GSE38382SRP013467PRJNA167771

REPOSITORIES: GEO, ArrayExpress, ENA

Similar Datasets

2012-11-30 | E-GEOD-38383 | ArrayExpress
| GSE85181 | GEO
| GSE85124 | GEO
| GSE85125 | GEO
| GSE85127 | GEO
| GSE93803 | GEO
| PRJNA167758 | ENA
2019-03-29 | PXD012606 | Pride
| PRJNA167772 | ENA
| PRJNA167771 | ENA