Transcriptomics

Dataset Information

358

Argonaute and Trimmer generate Dicer-independent priRNAs and nucleate RNAi


ABSTRACT: RNA interference is involved in silencing of transposable and repetitive elements. How these elements are initially recognized by RNAi is a fundamental and unanswered question. We previously identified a class of Dicer-independent small RNAs, called primal small RNAs (priRNAs), in fission yeast. The mechanism by which Dicer-independent small RNAs are generated is not clear for any species. Here we reconstitute priRNA biogenesis in vitro and demonstrate that priRNAs can nucleate RNAi in vivo. We identify 3'-5' exonuclease Trimmer and show that Argonaute, loaded with longer RNA precursors, recruits Trimmer to generate the new 3' end. Next, we show that antisense priRNAs accumulate in rrp6Δ cells and nucleate RNAi at subset of protein coding genes in a Trimmer- and priRNA-dependent manner. Thus, Rrp6-mediated degradation of antisense transcripts and priRNA precursors protects the genome from spurious RNAi. Our results suggest that Argonaute association with random RNA degradation products triggers RNAi in a process of transcriptome surveillance. small RNA profiling in wild type S. pombe cells and in mutant cells

ORGANISM(S): Schizosaccharomyces pombe  

SUBMITTER: Mario Halic  

PROVIDER: E-GEOD-38636 | ArrayExpress | 2014-04-21

SECONDARY ACCESSION(S): GSE38636SRP013641PRJNA168300

REPOSITORIES: GEO, ArrayExpress, ENA

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Publications

Argonaute and Triman generate dicer-independent priRNAs and mature siRNAs to initiate heterochromatin formation.

Marasovic Mirela M   Zocco Manuel M   Halic Mario M  

Molecular cell 20131003 2


RNAi is a conserved mechanism in which small RNAs induce silencing of complementary targets. We have previously identified priRNAs, a class of Dicer-independent small RNAs in fission yeast. The mechanism by which Dicer-independent small RNAs are generated is not well understood in any species. Here we reconstitute the final steps of priRNA and siRNA biogenesis in vitro. We identify the 3'-5' exonuclease Triman and demonstrate that Argonaute, loaded with longer RNA precursors, recruits Triman to  ...[more]

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