Transcriptomics

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Microarray analysis of At-med16-1 and wild type (Col-0) infected with the avirulent bacterial pathogen Pst DC3000/avrRpt2


ABSTRACT: The goal of the microarray experiment was to identify defense genes that were differentially expressed in the Arabidopsis mutant At-med16-1 and wild type in response to infection of the avirulent bacterial pathogen Pst DC3000/avrRpt2. Results indicated that, compared with the wild type, several positive regulators of SAR (systemic acquired resistance) were downregulated and a group of SAR negative regulators were upregulated in At-med16-1. Three biological replicates with leaves from 8 plants per sample were collected at 0, 4, 8, and 24 hours after inoculation with the avirulent bacterial pathogen Pst DC3000/avrRpt2. After extraction, RNA concentration was determined on a NanoDrop Spectrophotometer (Thermofisher Scientific, Waltham, MA) and sample quality was assessed using the 2100 Bioanalyzer (Agilent Technologies, Santa Clara, CA). Equal amount of RNA from the 3 biological replicates were pooled as one sample for microarray analysis.

ORGANISM(S): Arabidopsis thaliana  

SUBMITTER: Yijun Sun  Jin Yao   Yanping Zhang   Zhonglin Mou   Xudong Zhang    

PROVIDER: E-GEOD-38999 | ArrayExpress | 2012-06-29

SECONDARY ACCESSION(S): GSE38999PRJNA169570

REPOSITORIES: GEO, ArrayExpress

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