Transcriptomics

Dataset Information

3

Cohesin association to replication sites depends on Rad50 and promotes fork restart


ABSTRACT: The cohesin complex holds together newly-replicated chromatids and is involved in diverse pathways that preserve genome integrity. We show that in budding yeast, cohesin is transiently recruited to active replication origins and it spreads along DNA as forks progress. When DNA synthesis is impeded, cohesin accumulates at replication sites and is critical for the recovery of stalled forks. Cohesin enrichment at replication forks does not depend on H2A(X) formation, which differs from its loading requirements at DNA double-strand breaks (DSBs). However, cohesin localization is largely reduced in rad50delta mutants and cells lacking both Mec1 and Tel1 checkpoint kinases. Interestingly, cohesin loading at replication sites depends on the structural features of Rad50 that are important for bridging sister chromatids, including the CXXC hook domain and the length of the coiled-coil extensions. Together, these data reveal a novel function for cohesin in the maintenance of genome integrity during S phase. Scc1 ChIP, Rad50 ChIP and BrdU IP in wild type and mutants at different stages of the cell cycle.

ORGANISM(S): Saccharomyces cerevisiae W303  

SUBMITTER: Philippe Pasero   Armelle Lengronne  Marta B Davidson  John H Petrini  Julien Bacal  Jennifer A Cobb  Mireille Tittel-Elmer  Marcel Hohl  Philippe François 

PROVIDER: E-GEOD-39078 | ArrayExpress | 2012-10-01

SECONDARY ACCESSION(S): GSE39078PRJNA170884

REPOSITORIES: GEO, ArrayExpress

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