Proteomics

Dataset Information

8

Autoantibodies are abundant in all human sera: a demographic characterization via protein microarrays


ABSTRACT: Human serum samples were probed onto human protein microarrays in order to ascertain the number and diversity of naturally ocurring self-reactive IgG autoantibodies. In the study presented here, 166 human serum samples were probed onto human protein microarrays in order to identify self-reactive IgG autoantibodies and study their number and diversity in variable donor demographics. Microarray data were analyzed using several statistical programs, including Prospector v5.2, and autoantibodies with a relative fluorescence Z-factor value of greater than or equal to 0.4 were considered significant.

ORGANISM(S): Homo sapiens  

SUBMITTER: Cassandra DeMarshall   Nimish Acharya  Eric P Nagele  Robert G Nagele  Min Han 

PROVIDER: E-GEOD-39087 | ArrayExpress | 2013-04-25

SECONDARY ACCESSION(S): GSE39087PRJNA169922

REPOSITORIES: GEO, ArrayExpress

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Publications

Natural IgG autoantibodies are abundant and ubiquitous in human sera, and their number is influenced by age, gender, and disease.

Nagele Eric P EP   Han Min M   Acharya Nimish K NK   DeMarshall Cassandra C   Kosciuk Mary C MC   Nagele Robert G RG  

PloS one 20130402 4


The presence of self-reactive IgG autoantibodies in human sera is largely thought to represent a breakdown in central tolerance and is typically regarded as a harbinger of autoimmune pathology. In the present study, immune-response profiling of human serum from 166 individuals via human protein microarrays demonstrates that IgG autoantibodies are abundant in all human serum, usually numbering in the thousands. These IgG autoantibodies bind to human antigens from organs and tissues all over the b  ...[more]

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