Transcriptomics

Dataset Information

6

Gene expression profiling of cidofovir treatment and resistance inHeLa, HaCaT, and PHK cells


ABSTRACT: Cidofovir is an acyclic nucleoside phosphonate with strong antiviral activity against a broad spectrum of DNA viruses. Although it has previously been shown that cidofovir exerts an antiproliferative effect on HPV positive cells by the induction of apoptosis, the exact mechanism of action remains to be unraveled. In order to study the activity of cidofovir against HPV, gene expression profiling was performed in cidofovir-treated and cidofovir-resistant HeLa, HaCaT, and PHK cells by means of microarrays (HG-U133 Plus 2, Affymetrix). Controls (untreated HeLa, HaCaT, and PHK wild-type cells): 72h of growth. Cidofovir treated (50 µg/ml) HeLa, HaCaT, and PHK cells: 72h of growth. Cidofovir resistant cells (HeLa #52 and HaCaT #51): 72h of growth. All samples were performed in triplicates.

ORGANISM(S): Homo sapiens  

SUBMITTER: Joke Allemeersch   Tim De Schutter  Robert Snoeck  Dimitrios Topalis  Graciela Andrei 

PROVIDER: E-GEOD-39293 | ArrayExpress | 2013-07-03

SECONDARY ACCESSION(S): GSE39293PRJNA170506

REPOSITORIES: GEO, ArrayExpress

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Publications

Cidofovir selectivity is based on the different response of normal and cancer cells to DNA damage.

De Schutter Tim T   Andrei Graciela G   Topalis Dimitri D   Naesens Lieve L   Snoeck Robert R  

BMC medical genomics 20130523


BACKGROUND: Cidofovir (CDV) proved efficacious in treatment of human papillomaviruses (HPVs) hyperplasias. Antiproliferative effects of CDV have been associated with apoptosis induction, S-phase accumulation, and increased levels of tumor suppressor proteins. However, the molecular mechanisms for the selectivity and antitumor activity of CDV against HPV-transformed cells remain unexplained. METHODS: We evaluated CDV drug metabolism and incorporation into cellular DNA, in addition to whole genome  ...[more]

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