Genomics

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ChIP-seq using a Dp1 antibody in Drosophila S2R+ cells


ABSTRACT: Here we report the identification of genomic regions of DNA bound by Dp1 in Drosophila S2R+ cells. Dp1 is a dimerization partner of several E2F transcription factors and is needed for E2F target promoter binding. We find that Dp1 binds the promoter regions of genes important for oxidative phosphorylation. This result is important since our data demonstrates that expression of several oxidative phosphorylation genes is down-regulated in dDP mutant Drosophila 3rd instar larval eye imaginal discs. These ChIP-seq results suggest that the mechanism by which dDP regulates expression of these genes is direct. In addition, we have confirmed a number of these Dp1 bound gene promoters by conventional Chromatin Immunoprecipitation. Examination of Dp1 bound regions of genomic DNA in S2R+ cells.

ORGANISM(S): Drosophila melanogaster  

SUBMITTER: Maxim Frolov   Abul B Islam  Aaron Ambrus 

PROVIDER: E-GEOD-39393 | ArrayExpress | 2013-11-25

SECONDARY ACCESSION(S): SRP014425GSE39393PRJNA170768

REPOSITORIES: GEO, ArrayExpress, ENA

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Publications

Genome-wide analysis using ChIP to identify isoform-specific gene targets.

Beshiri Michael L ML   Islam Abul A   DeWaal Dannielle C DC   Richter William F WF   Love Jennifer J   Lopez-Bigas Nuria N   Benevolenskaya Elizaveta V EV  

Journal of visualized experiments : JoVE 20100707 41


Recruitment of transcriptional and epigenetic factors to their targets is a key step in their regulation. Prominently featured in recruitment are the protein domains that bind to specific histone modifications. One such domain is the plant homeodomain (PHD), found in several chromatin-binding proteins. The epigenetic factor RBP2 has multiple PHD domains, however, they have different functions (Figure 4). In particular, the C-terminal PHD domain, found in a RBP2 oncogenic fusion in human leukemia  ...[more]

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