Transcriptomics

Dataset Information

296

RNA-seq quantification of expression changes upon unc-62 knockdown in adult Caenorhabditis elegans.


ABSTRACT: We performed RNA-seq to quantify gene expression changes in adult worms upon knockdown of transcription factor unc-62/Homothorax. unc-62 is a developmental regulator that binds proximal to age-regulated transcripts and modulates lifespan. In the intestine (in which tissue-specific unc-62 knockdown increases lifespan), we identify multiple effects of unc-62 knockdown linked to extension of longevity. First, unc-62 RNAi decreases the expression of yolk proteins (vitellogenins) that aggregate in the body cavity and become toxic in old age. Second, unc-62 RNAi results in a broad increase in expression of intestinal genes that typically decrease expression with age, suggesting that unc-62 activity balances intestinal resource allocation between yolk protein expression and fertility on the one hand and somatic functions on the other. mRNA profiling by Illumina HiSeq of 3 biological replicates of day 4 adult Caenorhabditis elegans that were fed either control or unc-62 RNAi beginning at day 1 of adulthood.

ORGANISM(S): Caenorhabditis elegans  

SUBMITTER: Eric L Van Nostrand   Stuart K Kim 

PROVIDER: E-GEOD-39574 | ArrayExpress | 2012-09-01

SECONDARY ACCESSION(S): GSE39574SRP014544PRJNA171150

REPOSITORIES: GEO, ArrayExpress, ENA

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