Transcriptomics

Dataset Information

63

FoxA1 specifies unique androgen and glucocorticoid receptor binding events in prostate cancer cells (mRNA)


ABSTRACT: We report the androgen receptor recruitment to the chromatin of androgen responsive prostate cancer cell lines, LNCaP-1F5 and VCaP in response to physiological androgen 5a-dihydrotestosterone (DHT) using ChIP-sequencing. We compare the AR recruitment by DHT to that by partial agonist/antagonist cyproterone acetate and mifepristone (RU486) in LNCaP-1F5 cells. We also report the role of glucocorticoid receptor recruitment in presence of dexamethasone (Dex) in androgen responsive prostate cancer cells. The AR and GR cistrome analysis is subsequently compared with gene expression data and RNA Pol II analysis. The ChIP-seq has been performed using AR, GR, RNA Pol II antibodies. Expression profiling by microarray of LNCaP-1F5 cells treated with vehicle, 100 nM 5a-dihydrotestosterone (DHT), 100 nM dexamethasone (Dex), 1000 nM cyproterone acetate (CPA), 100 nM mifepristone (RU486) and combination of 100 nM DHT,100 nM Dex for 24 hours.

ORGANISM(S): Homo sapiens  

SUBMITTER: Biswajyoti Sahu   Päivi Pihlajamaa  Kristian Ovaska  Olli A. Jänne  Sampsa Hautaniemi  Ievgenii Sinielnikov  Olli A Jänne  Marko Laakso 

PROVIDER: E-GEOD-39654 | ArrayExpress | 2013-02-05

SECONDARY ACCESSION(S): GSE39654PRJNA171946

REPOSITORIES: GEO, ArrayExpress

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Publications

FoxA1 specifies unique androgen and glucocorticoid receptor binding events in prostate cancer cells.

Sahu Biswajyoti B   Laakso Marko M   Pihlajamaa Päivi P   Ovaska Kristian K   Sinielnikov Ievgenii I   Hautaniemi Sampsa S   Jänne Olli A OA  

Cancer research 20121226 5


The forkhead protein FoxA1 has functions other than a pioneer factor, in that its depletion brings about a significant redistribution in the androgen receptor (AR) and glucocorticoid receptor (GR) cistromes. In this study, we found a novel function for FoxA1 in defining the cell-type specificity of AR- and GR-binding events in a distinct fashion, namely, for AR in LNCaP-1F5 cells and for GR in VCaP cells. We also found different, cell-type and receptor-specific compilations of cis-elements enric  ...[more]

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