Dataset Information


Nitrogen-efficient rice over-expressing alanine aminotransferase

ABSTRACT: Oryza sativa cv. Nipponbare was engineered to over-express a barley alanine aminotransferase (alaAT) gene using the promoter (OsANT1) from a rice aldehyde dehydrogenase gene that expresses in roots. We are using biotechnology to improve the nitrogen use efficiency of rice by over-expressing alaAT in a tissue specific (root) manner. The AlaAT enzyme is a reversible aminotransferase that is linked to both C and N metabolism since it uses pyruvate plus glutamate to produce alanine and 2-oxoglutarate, and visa versa. Wildtype rice (Nipponbare) and three independent OsANT1:HvAlaAT rice transgenic lines (AGR1/7, AGR1/8 and AGR3/8) were grown hydroponically with 5mM NH4+ as the nitrogen source, to the reproductive stage. RNA samples were taken at active tillering, maximum tillering and end-of-tillering stages from root and shoot, at mid-day of the plants' day/night cycle. The RNA from root and shoot at maxiumum tillering was used for microarray analysis. Please read Beatty et al., 2009, Plant Biotechnology Journal 7, pp562-576 for further details..


ORGANISM(S): Oryza sativa  

SUBMITTER: Perrin H Beatty   Perrin Hudson Beatty  Ashok K Shrawat  Rebecka T Carroll  Allen G Good  Tong Zhu 

PROVIDER: E-GEOD-39687 | ArrayExpress | 2012-07-26



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Transcriptome analysis of nitrogen-efficient rice over-expressing alanine aminotransferase.

Beatty Perrin H PH   Shrawat Ashok K AK   Carroll Rebecka T RT   Zhu Tong T   Good Allen G AG  

Plant biotechnology journal 20090608 6

Crop plants require nitrogen for key macromolecules, such as DNA, proteins and metabolites, yet they are generally inefficient at acquiring nitrogen from the soil. Crop producers compensate for this low nitrogen utilization efficiency by applying nitrogen fertilizers. However, much of this nitrogen is unavailable to the plants as a result of microbial uptake and environmental loss of nitrogen, causing air, water and soil pollution. We engineered rice over-expressing alanine aminotransferase (Ala  ...[more]

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