Genomics

Dataset Information

0

Zygotic gene activatory network in zebrafish is globally controlled by Pou5f1 SoxB1 enhancers


ABSTRACT: Genetic control of pluripotent mammalian ES cells is determined by a transcriptional network, with a "central core" of transcription factors, Pou5f1, Sox2 and Nanog. Zebrafish homologues of the "core pluripotency factors" Pou5f1, SoxB1 and Nanog-like are also crucially involved in early development. However, the degree of functional similarity of the network between mammals and non-mammals is a matter of debate. To identify the components of Pou5f1-dependent transcriptional networks, we determined the genomic binding sites for Pou5f1 and Sox2 in late blastula stage zebrafish embryos using ChIP-seq. We found that Sox2 and Pou5f1 are co-binding to the regulatory regions of Sox2, Pou5f1, and Nanog-like, as well as to multiple orthologues of mammalian plutipotency network components. Deep sequencing was performed using the Illumina GAIIx on DNA samples obtained from Sox2 ChIP, Pou5f1-Flag ChIP and Input Control. Pou5f1 was analysed in technical duplicates to obtain higher sequencing depth.

ORGANISM(S): Danio rerio  

SUBMITTER: Julia Maes  Manuel Leichsenring   Rebecca Mössner   Daria Onichtchouk   Wolfgang Driever    

PROVIDER: E-GEOD-39780 | ArrayExpress | 2013-08-10

SECONDARY ACCESSION(S): GSE39780SRP014632PRJNA171706

REPOSITORIES: GEO, ArrayExpress, ENA

altmetric image

Publications

Sorry, this publication's infomation has not been loaded in the Indexer, please go directly to PUBMED or Altmetric.

Similar Datasets

2012-01-28 | E-GEOD-35373 | ArrayExpress
2013-07-31 | E-GEOD-41107 | ArrayExpress
2011-03-03 | E-GEOD-27535 | ArrayExpress
2014-11-14 | E-GEOD-55893 | ArrayExpress
2012-01-28 | GSE35373 | GEO
2016-06-24 | E-GEOD-76230 | ArrayExpress
2013-09-22 | E-GEOD-47558 | ArrayExpress
2008-11-25 | BIOMD0000000203 | BioModels Database
2008-11-26 | BIOMD0000000204 | BioModels Database
2011-09-12 | E-GEOD-28782 | ArrayExpress