Dataset Information


FoxA1 specifies unique androgen and glucocorticoid receptor binding events in prostate cancer cells (HTS)

ABSTRACT: We report the androgen receptor recruitment to the chromatin of androgen responsive prostate cancer cell lines, LNCaP-1F5 and VCaP in response to physiological androgen 5a-dihydrotestosterone (DHT) using ChIP-sequencing. We compare the AR recruitment by DHT to that by partial agonist/antagonist cyproterone acetate (CPA), mifepristone (RU486) and bicalutamide (Bica) in LNCaP-1F5 cells. We also report the role of glucocorticoid receptor recruitment in presence of dexamethasone (Dex) in androgen responsive prostate cancer cells. The AR and GR cistrome analysis is subsequently compared with gene expression data and RNA Pol II analysis. The ChIP-seq has been performed using AR, GR, RNA Pol II antibodies. Examination of AR and GR binding sites in LNCaP-1F5 and VCaP cells in presence of DHT and Dex respectively. Further analysis of AR binding sites in LNCaP-1F5 cells treated with partial agonist/antagonists, CPA, RU486 and Bica. Additionally RNA Pol II mapping is performed in cells treated with DHT and Dex.

ORGANISM(S): Homo sapiens  

SUBMITTER: Hautaniemi Sampsa   Sahu Biswajyoti  Olli A. Jänne  Ovaska Kristian  Pihlajamaa Paivi  Sinielnikov Ievgenii  Laakso Marko  Jänne A Olli 

PROVIDER: E-GEOD-39879 | ArrayExpress | 2013-02-05



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FoxA1 specifies unique androgen and glucocorticoid receptor binding events in prostate cancer cells.

Sahu Biswajyoti B   Laakso Marko M   Pihlajamaa Päivi P   Ovaska Kristian K   Sinielnikov Ievgenii I   Hautaniemi Sampsa S   Jänne Olli A OA  

Cancer research 20121226 5

The forkhead protein FoxA1 has functions other than a pioneer factor, in that its depletion brings about a significant redistribution in the androgen receptor (AR) and glucocorticoid receptor (GR) cistromes. In this study, we found a novel function for FoxA1 in defining the cell-type specificity of AR- and GR-binding events in a distinct fashion, namely, for AR in LNCaP-1F5 cells and for GR in VCaP cells. We also found different, cell-type and receptor-specific compilations of cis-elements enric  ...[more]

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