Genomics

Dataset Information

298

Dnmt2-dependent methylomes lack biologically relevant DNA methylation patterns [Schistosoma mansoni]


ABSTRACT: Dnmt2 is a widely conserved protein, which is closely related to eukaryotic DNA methyltransferases. However, Dnmt2 shows a robust tRNA methyltransferase activity and only limited activity towards DNA. Interestingly, a recent study has provided evidence for a biologically important function of Dnmt2-dependent DNA methylation in the blood fluke Schistosoma mansoni, which seemed to contradict the weak activity of the enzyme in other organisms. We now used whole-genome bisulfite sequencing to comprehensively analyze the methylome of adult worms and could not detect any evidence for biologically relevant DNA methylation patterns. We also characterized the methylome of Drosophila melanogaster embryos and did not find any evidence for DNA methylation. Unconverted cytosine residues were detectable only at very low levels and shared many attributes with bisulfite deamination artifacts. Our results thus strongly argue against a DNA methyltransferase activity of Dnmt2 and suggest that Dnmt2-dependent phenotypes are caused by reduced tRNA methylation. Whole genome methylation analysis of S. mansoni. One sample was analyzed, containing DNA from adult male worms.

ORGANISM(S): Schistosoma mansoni  

SUBMITTER: F Lyko   M Fantappie  Guenter Raddatz 

PROVIDER: E-GEOD-39997 | ArrayExpress | 2013-05-06

SECONDARY ACCESSION(S): SRP014761GSE39997PRJNA172334

REPOSITORIES: GEO, ArrayExpress, ENA

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Dnmt2-dependent methylomes lack defined DNA methylation patterns.

Raddatz Günter G   Guzzardo Paloma M PM   Olova Nelly N   Fantappié Marcelo Rosado MR   Rampp Markus M   Schaefer Matthias M   Reik Wolf W   Hannon Gregory J GJ   Lyko Frank F  

Proceedings of the National Academy of Sciences of the United States of America 20130502 21


Several organisms have retained methyltransferase 2 (Dnmt2) as their only candidate DNA methyltransferase gene. However, information about Dnmt2-dependent methylation patterns has been limited to a few isolated loci and the results have been discussed controversially. In addition, recent studies have shown that Dnmt2 functions as a tRNA methyltransferase, which raised the possibility that Dnmt2-only genomes might be unmethylated. We have now used whole-genome bisulfite sequencing to analyze the  ...[more]

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