Transcriptomics

Dataset Information

3

Mesenchymal stem cells reduce inflammation while enhancing bacterial clearance and improving survival in sepsis.


ABSTRACT: Expression data from Total RNA extracted from murine spleen, liver, lungs, kidneys and hearts. Sepsis was induced in C57Bl/6J mice by cecal ligation and puncture (CLP), followed 6 hours later by an intravenous injection of Mesenchymal Stem Cell (MSC) or saline. Twenty-eight hours after CLP, plasma, bronchoalveolar lavage (BAL) fluid and tissues were collected for analyses. Total RNA was extracted using Trizol (as per manufactures' instruction) followed by clean-up procedure using Qiagen RNA easy Prep (as per manufactures instructions) In the following study we hypothesized that mesenchymal stem cells (MSCs), which have documented immunomodulatory properties, would reduce sepsis-associated inflammation and organ injury in a clinically relevant model of sepsis. To identify the molecular changes associated with decreased inflammation in CLP-injured mice treated with MSCs, we analyzed the gene expression profiles from spleens, liver, lungs, kidneys and heart collected at 28 hours from 4 animals per group: sham/saline, CLP/saline, and CLP/MSCs.

ORGANISM(S): Mus musculus  

SUBMITTER: Jack J Haitsma   Duncan J Stewart  Patrick F Lai  Yupu Deng  Arthur S Slutsky  W C Liles  Claudia Chimisso dos Santos  Claudia C Dos Santos  Shirley H Mei 

PROVIDER: E-GEOD-40180 | ArrayExpress | 2012-12-31

SECONDARY ACCESSION(S): GSE40180PRJNA173022

REPOSITORIES: GEO, ArrayExpress

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