Genomics

Dataset Information

0

Genome-wide targets of conserved histone chaperone Asf1 in budding yeast


ABSTRACT: Asf1, through its histone chaperone activity, helps chromatin closing/opening during DNA replication, repair, recombination and transcription. Despite extensive research on Asf1-mediated physiological functions, a genome-wide localization map is lacking, limiting our knowledge of chromosomal features targeted by Asf1. We present a high-resolution genome-wide map of Asf1, localizing at essentially all pol III-transcribed genes, highly active pol II-transcribed genes and heterochromatic features. Pol III-transcribed genes are negatively regulated by Asf1, whereas pol II genes are regulated indirectly by Asf1-dependent H3K56 acetylation. Interestingly, Asf1 localization along yeast chromosomes shows nearly identical distribution to that of the condensin complex, predicting a functional overlap in chromosome architecture and genome organization. ChIP-seq analysis of Asf1 targets using a yeast strain that expresses an 18-Myc tag fused to the C-terminus of ASF1. Two biological replicates and one mock/control were performed. The Illumina GAII was used. ChIP-seq reads are aligned to the budding yeast sacCer3 (2011) assembly.

ORGANISM(S): Saccharomyces cerevisiae  

SUBMITTER: Pooran Singh Dewari   Pooran S Dewari  Purnima Bhargava 

PROVIDER: E-GEOD-40717 | ArrayExpress | 2014-07-30

SECONDARY ACCESSION(S): SRP015669GSE40717PRJNA174746

REPOSITORIES: GEO, ArrayExpress, ENA

Similar Datasets

2013-09-05 | E-GEOD-42714 | ArrayExpress
2015-08-12 | E-GEOD-60273 | ArrayExpress
2014-03-08 | E-GEOD-55703 | ArrayExpress
2010-04-14 | E-GEOD-20309 | ArrayExpress
2014-11-03 | E-GEOD-53420 | ArrayExpress
2010-04-25 | E-GEOD-20040 | ArrayExpress
2013-06-21 | E-GEOD-39566 | ArrayExpress
2010-04-25 | E-GEOD-20071 | ArrayExpress
| GSE28098 | GEO
2010-04-14 | E-GEOD-20609 | ArrayExpress