Genomics

Dataset Information

300

ChIPseq analysis of IRF4 and BATF in immune cells


ABSTRACT: Purpose: The purpose of this study is to find the binding partner of IRF4 in the context of Th17- cell differentiation. To this end, we have used ChIPseq analysis followed by de novo motif search around genome-wide binding sites to identify BATF as the binding partner for IRF4 in the context of not only Th17 cells but other immune cell types as well. Naïve T-cells isolated from the spleen of C57BL/6J mice are cultured under Th17, Th2 or Th0 polarizing conditions for 42 hrs and subject to ChIP using IRF4 and/or BATF antibodies followed by high-throughput sequencing. Bone marrow derived dendritic cells (BMDCs) were stimulated with LPS for 6hrs and similrly subjected to ChIPseq analysis with IRF4.

ORGANISM(S): Mus musculus  

SUBMITTER: Wenwen Zeng   Smita Agrawal  Bryan Lugt 

PROVIDER: E-GEOD-40727 | ArrayExpress | 2012-09-13

SECONDARY ACCESSION(S): SRP015676GSE40727PRJNA174767

REPOSITORIES: GEO, ArrayExpress, ENA

Similar Datasets

2012-09-13 | E-GEOD-40483 | ArrayExpress
2013-12-22 | E-GEOD-52772 | ArrayExpress
2012-09-18 | E-GEOD-39756 | ArrayExpress
| GSE39756 | GEO
2014-03-02 | E-GEOD-54191 | ArrayExpress
| GSE86535 | GEO
2013-12-03 | E-GEOD-40623 | ArrayExpress
2014-06-03 | E-GEOD-56857 | ArrayExpress
| GSE85172 | GEO
| GSE85173 | GEO