Transcriptomics

Dataset Information

29

PPARδ activation in human myotubes increases mitochondrial fatty acid oxidative capacity and reduces glucose utilization by a switch in substrate preference.


ABSTRACT: The role of peroxisome proliferator-activated receptor δ (PPARδ) activation on global gene expression and mitochondrial fuel utilization were investigated in human myotubes. Only 21 genes were up-regulated and 3 genes were down-regulated after activation by the PPARδ agonist GW501516. Pathway analysis showed up-regulated mitochondrial fatty acid oxidation, TCA cycle and cholesterol biosynthesis. GW501516 increased oleic acid oxidation and mitochondrial oxidative capacity by 2-fold. Glucose uptake and oxidation were reduced, but total substrate oxidation was not affected, indicating a fuel switch from glucose to fatty acid. Cholesterol biosynthesis was increased, but lipid biosynthesis and mitochondrial content were not affected. This study confirmed that the principal effect of PPARδ activation was to increase mitochondrial fatty acid oxidative capacity. Our results further suggest that PPARδ activation reduced glucose utilization through a switch in mitochondrial substrate preference by up-regulating pyruvate dehydrogenase kinase isozyme 4 and genes involved in lipid metabolism and fatty acid oxidation. Keywords: Expression profiling by array Human myotubes from four donors were exposed to a PPARδ agonist or control for 96 h after which gene expression was profiled.

ORGANISM(S): Homo sapiens  

SUBMITTER: Eili T Kase  G H Thoresen   Mark V Boekschoten   Arild C Rustan   Yuan Z Feng   Sander Kersten   Siril S Bakke   Guido Hooiveld   Natasa Nikolic    

PROVIDER: E-GEOD-40789 | ArrayExpress| 2014-06-03

SECONDARY ACCESSION(S): GSE40789PRJNA174969

REPOSITORIES: GEO, ArrayExpress

altmetric image

Publications

PPARδ activation in human myotubes increases mitochondrial fatty acid oxidative capacity and reduces glucose utilization by a switch in substrate preference.

Feng Yuan Z YZ   Nikolić Nataša N   Bakke Siril S SS   Boekschoten Mark V MV   Kersten Sander S   Kase Eili T ET   Rustan Arild C AC   Thoresen G Hege GH  

Archives of physiology and biochemistry 20130830 1


The role of peroxisome proliferator-activated receptor δ (PPARδ) activation on global gene expression and mitochondrial fuel utilization were investigated in human myotubes. Only 21 genes were up-regulated and 3 genes were down-regulated after activation by the PPARδ agonist GW501516. Pathway analysis showed up-regulated mitochondrial fatty acid oxidation, TCA cycle and cholesterol biosynthesis. GW501516 increased oleic acid oxidation and mitochondrial oxidative capacity by 2-fold. Glucose uptak  ...[more]

Similar Datasets

2014-05-01 | E-GEOD-11936 | ArrayExpress
2012-06-21 | E-GEOD-38590 | ArrayExpress
2014-01-15 | BIOMD0000000506 | BioModels Database
2014-01-15 | BIOMD0000000505 | BioModels Database
2013-10-28 | E-GEOD-45554 | ArrayExpress
2014-04-03 | E-GEOD-43832 | ArrayExpress
2012-06-25 | E-GEOD-32357 | ArrayExpress
2013-01-15 | E-GEOD-37897 | ArrayExpress
2012-06-25 | E-GEOD-31901 | ArrayExpress
2012-03-27 | E-GEOD-18589 | ArrayExpress