Transcriptomics

Dataset Information

4

The effect of ACSL4 expression on overall gene expression in breast cancer cell lines


ABSTRACT: ACSL4 expression appears to be inversely associated with steroid hormone and growth factor receptor expression in breast cancer and positively correlated with an aggressive breast cancer phenotype. Neither MCF-7 nor SKBr3 cells normally express ACSL4, and when manipulated to do so, develop basal-like characteristics, including increased proliferation, migration and anchorage independent growth. We used an Affymetrix array platform to assess changes in individual gene expression as a function of conditional and stable expression of ACSL4 in MCF-7 and SKBr3 cells. In experiment 1, MCF-7 Tet-ON cells were transfected with ACSL4 cDNA and induced to express ACSL4 by addition of 1ug/ml of doxycycline (A) or vehicle (C) for 72 hours. Three controls (C) and three doxycycline-induced (A) samples were evaluated for gene expression. In experiment 2, MCF-7 cells were stably transfected with either an empty vector (C) or Lenti-ORF-ACSL4 (A). In experiment 3, SKBr3 cells were stably transfected with either an empty vector (C) or Lenti-ORF-ACSL4 (A).

REANALYSED by: E-GEOD-40968

ORGANISM(S): Homo sapiens  

SUBMITTER: Sylvia Adams   Yirong Li  Max T Marcus  David Y Zhang  Xinyu Wu  Fei Ye  Garrett Daniels  Ling H Wang  Xin Wen  Baljit Singh  Jinhua Wang  Peng Lee  Marie E Monaco  Jiri Zavadil 

PROVIDER: E-GEOD-40968 | ArrayExpress | 2012-09-19

SECONDARY ACCESSION(S): GSE40968PRJNA175454

REPOSITORIES: GEO, ArrayExpress

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