Transcriptomics

Dataset Information

4

Chip-chip from Escherichia coli MG1655 K-12, WT and ∆fnr strains


ABSTRACT: Mapping the occupancy of FNR, HNS, and IHF throughout the genome of Escherchia coli MG1655 K-12 using an affinity purified antibody under anerobic growth conditions. We also mapped the binding of the ß subunit of RNA Polymerase under both aerobic and anaerobic growth conditions. As a control, we also performed ChIP-chip on FNR in a ∆fnr mutant strain of Escherchia coli MG1655 K-12. We also examined FNR immunoprecipitation at various FNR concentrations using IPTG and Ptac::fnr (PK8263). The ∆hns/∆stpA strains were also used. Descirbed in the manuscript Genome-scale Analysis of E. coli FNR Reveals the Complexity of Bacterial Regulon Structure Mapping of occupancy of FNR, NNS, IHF and ß of RNAP in the genome of Escherchia coli MG1655 K-12 under aerobic or anaerobic growth conditions. Immunoprecipitated DNA compared to INPUT for each sample.

ORGANISM(S): Escherichia coli str. K-12 substr. MG1655  

SUBMITTER: Kevin Myers   Patricia Kiley  Dongjun Chung  Robert Landick  Huihuang Yan  Sunduz Keles  Irene Ong  Frances Tran  Kun Liang 

PROVIDER: E-GEOD-41186 | ArrayExpress | 2013-06-20

SECONDARY ACCESSION(S): GSE41186PRJNA176148

REPOSITORIES: GEO, ArrayExpress

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