Transcriptomics

Dataset Information

2

Transcription profiling of copper-stressed Pseudomonas aeruginosa


ABSTRACT: Anthropogenic pollution has increased the levels of heavy metals in the environment. Bacterial populations continue to thrive in highly polluted environments and bacteria must have mechanisms to counter heavy metal stress. We chose to examine the response of the environmentally-relevant organism Pseudomonas aeruginosa to two different copper treatments. A short, 45 min exposure to copper was done in the Cu shock treatment to examine the immediate transcriptional profile to Cu stress. The Cu adapted treatment was designed to view the transcriptional profile of cells that were actively growing in the presence of Cu. Experiment Overall Design: We analyzed 2 biological replicates of Pseudomonas aeruginosa exposed to a 45 min Cu shock as compared to a control that was exposed to HCl to bring the pH to similar levels. We analyzed 2 biological replicates of Pseudomonas aeruginosa that were grown in the presence of Cu for approx. 6h (Cu adapted) as compared to an untreated control.

ORGANISM(S): Pseudomonas aeruginosa  

SUBMITTER: Gail M. Teitzel  

PROVIDER: E-GEOD-4152 | ArrayExpress | 2007-10-24

SECONDARY ACCESSION(S): GSE4152GDS2377PRJNA94999

REPOSITORIES: GEO, ArrayExpress

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Publications

Survival and growth in the presence of elevated copper: transcriptional profiling of copper-stressed Pseudomonas aeruginosa.

Teitzel Gail M GM   Geddie Ashley A   De Long Susan K SK   Kirisits Mary Jo MJ   Whiteley Marvin M   Parsek Matthew R MR  

Journal of bacteriology 20061001 20


Transcriptional profiles of Pseudomonas aeruginosa exposed to two separate copper stress conditions were determined. Actively growing bacteria subjected to a pulse of elevated copper for a short period of time was defined as a "copper-shocked" culture. Conversely, copper-adapted populations were defined as cells actively growing in the presence of elevated copper. Expression of 405 genes changed in the copper-shocked culture, compared to 331 genes for the copper-adapted cultures. Not surprisingl  ...[more]

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