Dataset Information


ChIP-seq analysis of CTCF binding sites in lymphocyte subsets [DP thymocytes]

ABSTRACT: In this study, we compare the binding pattern of CTCF at the T cell receptor alpha locus in different subsets of developing lymphocytes For the ChIP-seq, input and immunoprecipitated DNA was given to The Scripps DNA Array Facility, where it was prepared for massively parallel sequencing on Illumina Genome Analyzer IIx.

ORGANISM(S): Mus musculus  

SUBMITTER: Ali Torkamani   Jiyoti Verma-Gaur  Ann J Feeney 

PROVIDER: E-GEOD-41740 | ArrayExpress | 2012-12-03



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Tcra gene recombination is supported by a Tcra enhancer- and CTCF-dependent chromatin hub.

Shih Han-Yu HY   Verma-Gaur Jiyoti J   Torkamani Ali A   Feeney Ann J AJ   Galjart Niels N   Krangel Michael S MS  

Proceedings of the National Academy of Sciences of the United States of America 20121119 50

Antigen receptor locus V(D)J recombination requires interactions between widely separated variable (V), diversity (D), and joining (J) gene segments, but the mechanisms that generate these interactions are not well understood. Here we assessed mechanisms that direct developmental stage-specific long-distance interactions at the Tcra/Tcrd locus. The Tcra/Tcrd locus recombines Tcrd gene segments in CD4(-)CD8(-) double-negative thymocytes and Tcra gene segments in CD4(+)CD8(+) double-positive thymo  ...[more]

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