Transcriptomics

Dataset Information

2

Temporal gene expression analysis of the Brucella melitensis-infected bovine Peyer's patch


ABSTRACT: In a time course study, we characterized global gene expression profile of B. melitensis-infected bovine Peyer patches in the first 4 h p.i. Microarray analysis revealed that 2,916 bovine genes were detected as differentially expressed (z-score p < 0.025) in loops inoculated with virulent B. melitensis 16M compared with controls between 15 min and 4 h post-infection. From these genes, 2,286 (78%) were up- and 630 (22%) were down-regulated. Specific genes and biological processes identified in this study will further help elucidate how both host and Brucella interact during the early infectious process to the eventual benefit of the pathogen and to the detriment of the naïve host. Microarrays were used to examine the transcriptional profiles of bovine intestinal Peyer patches infected with wild type Brucella melitensis 16M across five time points (15 min, 30 min, 1, 2 and 4 hours). Intestinal loops inoculated with cell culture medium were used as a control. Experiments were performed in quadruplicate (bovine ligated ileal loops surgeries were performed with four calves), generating a total of 40 arrays.

ORGANISM(S): Bos taurus  

SUBMITTER: Robin E Everts   Jairo E Nunes  Kenneth Drake  Leslie Garry Adams  Carlos A Rossetti  L G Adams  Sangeeta Khare  Prasad Siddavatam  Tamara Gull  Harris A Lewin  Sara D Lawhon 

PROVIDER: E-GEOD-41835 | ArrayExpress | 2013-10-15

SECONDARY ACCESSION(S): GSE41835PRJNA178292

REPOSITORIES: GEO, ArrayExpress

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