Transcriptomics

Dataset Information

61

Real-time quantitative PCR analysis of microRNA in keratinocytes and reconstituted epidermis


ABSTRACT: Analysis of microRNA expression in keratinocytes by relative quantification (RQ) using the comparative Ct method. Real-time quantitative PCR analysis of microRNA in keratinocytes or reconstituted epidermis exposed to IL-22, LPS or UV radiation or a small molecule drug. Keratinocytes or reconstituted human epidermis were exposed to indicated treatments. A qPCR analysis of 365 microRNAs in keratinocytes. RNA (20 ng per reaction) reverse transcribed using 8 x Multiplex RT pools. Each resulting cDNA pool was mixed with TaqMan Universal PCR Master Mix and loaded in corresponding port on the TaqMan Array.

ORGANISM(S): Homo sapiens  

SUBMITTER: Kehinde Ross  

PROVIDER: E-GEOD-41838 | ArrayExpress | 2013-05-23

SECONDARY ACCESSION(S): GSE41838PRJNA178364

REPOSITORIES: GEO, ArrayExpress

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Publications

Expression of microRNA-184 in keratinocytes represses argonaute 2.

Roberts Julian C JC   Warren Richard B RB   Griffiths Christopher E M CE   Ross Kehinde K  

Journal of cellular physiology 20131201 12


Interleukin-22 (IL-22) is a proinflammatory cytokine that has been associated with the pathogenesis of inflammatory skin disorders. However, the impact of IL-22 on microRNA (miRNA) expression in epidermal keratinocytes is unknown. Here we show that IL-22 induces miR-184 in reconstituted human epidermis (RHE) and in the HaCaT keratinocyte cell line. Exposure to IL-22 increased miR-184 expression 8- and 15-fold in RHE and HaCaT cells, respectively. Oncostatin M, an unrelated proinflammatory cytoki  ...[more]

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