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Longitudinal expression data from CD8+ T cells responding to LCMV-Armstrong or LCMV-Clone 13

ABSTRACT: During acute viral infections, naïve CD8+ T cells differentiate into effector CD8+ T cells and, after viral control, into memory CD8+ T cells. Memory CD8+ T cells are highly functional, proliferate rapidly upon reinfection and persist long-term without antigen. In contrast, during chronic infections, CD8+ T cells become “exhausted” and have poor effector function, express multiple inhibitory receptors, possess low proliferative capacity, and cannot persist without antigen. To compare the development of functional memory T cells with poorly functional exhausted T cells, we generated longitudinal transcriptional profiles for each. Naive CD44Lo CD8+ T cells were isolated and sorted from uninfected C57BL/6 mice and H2-Db GP33-specific CD8+ T cells were sorted using MHC-I tetramers at d6, 8, 15, and 30 p.i. with either LCMV Arm or LCMV clone 13. RNA from these CD8+ T cells was processed, amplified, labeled, and hybridized to Affymetrix GeneChip MoGene 1.0 st microarrays


ORGANISM(S): Mus musculus  

SUBMITTER: E J Wherry   E John Wherry  Alison Crawford  Jill Angelosanto 

PROVIDER: E-GEOD-41867 | ArrayExpress | 2012-11-23



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Network analysis reveals centrally connected genes and pathways involved in CD8+ T cell exhaustion versus memory.

Doering Travis A TA   Crawford Alison A   Angelosanto Jill M JM   Paley Michael A MA   Ziegler Carly G CG   Wherry E John EJ  

Immunity 20121115 6

Exhausted CD8(+) T cells function poorly and are negatively regulated by inhibitory receptors. Transcriptional profiling has identified gene expression changes associated with exhaustion. However, the transcriptional pathways critical to the differences between exhausted and functional memory CD8(+) T cells are unclear. We thus defined transcriptional coexpression networks to define pathways centrally involved in exhaustion versus memory. These studies revealed differences between exhausted and  ...[more]

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