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ABSTRACT: The expression profiles of Drosophila Kc167 and SL2 cells were compared on the CDMC_Drosophila_7k2 array platform. Three independent RNA samples from each cell line were isolated and compared in a pairwise fashion. Dye-flip experiments were also performed. See Neal et al. 2003 for results and discussion. Keywords = Kc167 Keywords = SL2 Keywords = Drosophila

ORGANISM(S): Drosophila melanogaster  

SUBMITTER: Anthony K So   Scott J Neal  Meredith L Gibson  Jon T Westwood 

PROVIDER: E-GEOD-419 | ArrayExpress | 2010-06-05



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Construction of a cDNA-based microarray for Drosophila melanogaster: a comparison of gene transcription profiles from SL2 and Kc167 cells.

Neal Scott J SJ   Gibson Meredith L ML   So Anthony K-C AK   Westwood J Timothy JT  

Genome 20031001 5

We have constructed a DNA microarray that represents approximately 6900 of the estimated 13,598 genes in the Drosophila melanogaster genome. The microarray contains 5756 target cDNAs from the Berkeley Drosophila Genome Project, 1078 cDNAs from the National Institutes of Health Drosophila testis cDNA library, and 546 gene fragments that were amplified from genomic DNA. The methods for DNA amplification and microarray manufacture are presented. Academic researchers can obtain the microarray from t  ...[more]

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