Transcriptomics

Dataset Information

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In vivo NCL-targeting affects breast cancer aggressiveness through miRNA regulation [Affymetrix]


ABSTRACT: Numerous studies have described the altered expression and the causal role of miRNAs in human cancer. However, to date efforts to modulate miRNA levels for therapeutic purposes have been challenging to implement. Here, we find that Nucleolin (NCL), a major nucleolar protein, post-transcriptionally regulates the expression of a specific subset of miRNAs, including miR-21, miR-221, miR-222, and miR-103, causally involved in breast cancer initiation, progression and drug-resistance. We also show that NCL is commonly overexpressed in human breast tumors, and its expression correlates with that of NCL-dependent miRNAs. Finally, this study indicates that NCL-binding guanosine-rich aptamers affect the levels of NCL-dependent miRNAs and their target genes, reducing breast cancer cell aggressiveness, both in vitro and in vivo. These findings illuminate a path to novel therapeutic approaches based on NCL-targeting aptamers for the modulation of miRNA expression in the treatment of breast cancer. MCF7 cells were treated with the control drug or AS1411 aptamer. After 72hours total RNA was collected and analyzed by Affymetrix U133 plus.

REANALYSED by: E-GEOD-41972

ORGANISM(S): Homo sapiens  

SUBMITTER: Jia You   Jessica Consiglio  Luciano Cascione  Jingwen Guan  Pierluigi Gasparini  Alberto Rocci  Vincenzo Coppola  Luciana De Luca  Alessandro Lagana  Claudia Pivan  Guido Marcucci  Charles L Shapiro  Michael A Freitas  Tiffany Talabere  Flavia Pichiorri  Veronica Balatti  Carlo M Croce  Stefano Volinia  John C Byrd  Craig C Hofmeister  Dario Palmieri 

PROVIDER: E-GEOD-41972 | ArrayExpress | 2012-11-02

SECONDARY ACCESSION(S): GSE41972PRJNA178628

REPOSITORIES: GEO, ArrayExpress

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Publications


Numerous studies have described the altered expression and the causal role of microRNAs (miRNAs) in human cancer. However, to date, efforts to modulate miRNA levels for therapeutic purposes have been challenging to implement. Here we find that nucleolin (NCL), a major nucleolar protein, posttranscriptionally regulates the expression of a specific subset of miRNAs, including miR-21, miR-221, miR-222, and miR-103, that are causally involved in breast cancer initiation, progression, and drug resist  ...[more]

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