Transcriptomics

Dataset Information

4

The innate and adaptive immune response to BCG stimulation in splenocytes taken from C57BL/6 mice


ABSTRACT: The aim of this experiment was to investigate differential gene expression in splenocytes stimulated with BCG from naïve and BCG vaccinated mice. The differences between naïve and BCG vaccinated mice might indicate the mechanisms by which BCG vaccination confers an enhanced ability of splenocytes from BCG vaccinated mice to inhibit growth of BCG in splenocyte cultures as compared with splenocytes from naive animals. Splenocytes from 4 naïve and 4 BCG vaccinated female C57bl/6 mice were cultured for 12 hours with BCG (MOI 1:1) or without (unstimulated control). Each animal had a stimulated and an unstimulated sample hybridised to a beadchip.

ORGANISM(S): Mus musculus  

SUBMITTER: Helen McShane   leanne marsay  Helen E Fletcher  Leanne Marsay  Magali Kostov 

PROVIDER: E-GEOD-42090 | ArrayExpress | 2013-06-01

SECONDARY ACCESSION(S): GSE42090PRJNA179104

REPOSITORIES: GEO, ArrayExpress

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Publications

Mycobacterial growth inhibition in murine splenocytes as a surrogate for protection against Mycobacterium tuberculosis (M. tb).

Marsay Leanne L   Matsumiya Magali M   Tanner Rachel R   Poyntz Hazel H   Griffiths Kristin L KL   Stylianou Elena E   Marsh Philip D PD   Williams Ann A   Sharpe Sally S   Fletcher Helen H   McShane Helen H  

Tuberculosis (Edinburgh, Scotland) 20130531 5


Development of an improved vaccine against tuberculosis (TB) is hindered by the lack of a surrogate of protection. Efficacy of new TB vaccines in humans can only be evaluated by expensive and time consuming efficacy trials within TB endemic areas. It is critical that vaccines with the greatest potential to protect are selected for these trials. Mycobacterial growth inhibition assays (MGIAs) have been developed with the hope that these in-vitro functional assays will correlate with protection, wh  ...[more]

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