Transcriptomics

Dataset Information

4

Effects of EVI1 and EVI1Δ324 mild expression in HeLa cells


ABSTRACT: We studied the variations of mRNA amounts after Flag-EVI1, Flag-EVI1Δ324, or Flag expression in HeLa cells. Despites EVI1 discovery in 1988, its recognized role as a dominant oncogene in myeloid leukemia and more recently in epithelial cancers, only a few target genes were known and it was not clear why EVI1 was involved in cancer progression. Here we obtained the genomic binding occupancy and expression data for EVI1 in human cells. We identified numerous EVI1 target cancer genes and genes controlling cell migration and adhesion. Moreover, we characterized a transcriptional cooperation between AP1 and EVI1 that regulated proliferation and adhesion through a feed-forward loop. This study provides human genome-wide mapping and expression analyses for EVI1 that will be useful for the research community. 12 samples were collected. Each condition was done in 4 replicates, collected 24 hours after transfection (for mild expression of EVI1 or EVI1Δ324). Transfections with Flag-expressing vector were used as controls.

REANALYSIS of: GSE25213

ORGANISM(S): Homo sapiens  

SUBMITTER: Ahmed Sayadi   Neal G Copeland  Emilie A Bard-Chapeau  Guillaume Bourque  Justin Jeyakani  A Sayadi 

PROVIDER: E-GEOD-42251 | ArrayExpress | 2012-11-14

SECONDARY ACCESSION(S): GSE42251PRJNA179443

REPOSITORIES: GEO, ArrayExpress

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