Transcriptomics

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Expression data from mouse lung treated with saline or bleomycin


ABSTRACT: Lung fibrosis represents the final common pathway of a variety of lung injuries, and it is characterized, independently of etiology, by the expansion of the fibroblast/myofibroblast population and by the abnormal accumulation of extracellular matrix (ECM) replacing normal functional parenchyma. In general, there is no effective therapy, and currently the only helpful available treatment for advanced lung fibrosis is transplantation. In this study we aimed to document the time-related reversibility of bleomycin-induced lung fibrosis, and to determine the gene expression patterns associated with the initial progression and the subsequent regression of the fibrotic response by microarray in this bleomycin mouse model of lung fibrosis. Mice (C57BL/6 ) aging 8 to 10 weeks were instilled intratracheally with 0.10 U/10 g of bleomycin (Blenoxane, Bristol-Myers Squibb Co.) in 50 μl sterile saline. Control animals received an equal volume of sterile saline (9, 10). Mice were housed in specific pathogen-free conditions and provided with food and water ad libitum. All studies and procedures were approved by the Science and Ethic Committee at the National Institute of Respiratory Diseases and were performed according to the protocols and guidelines of the institutional animal care and use committee. Mice were sacrificed at 7 days and 1, 2, 3, and 4 months after bleomycin or saline treatment. For every variable, six control and six experimental mice were used.

ORGANISM(S): Mus musculus  

SUBMITTER: Annie Pardo  Naftali Kaminski   Sandra Cabrera   Moises Selman   Thomas Richards   Kazuhisa Konishi    

PROVIDER: E-GEOD-42301 | ArrayExpress | 2013-03-11

SECONDARY ACCESSION(S): GSE42301PRJNA180999

REPOSITORIES: GEO, ArrayExpress

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Publications

Gene expression profiles reveal molecular mechanisms involved in the progression and resolution of bleomycin-induced lung fibrosis.

Cabrera Sandra S   Selman Moises M   Lonzano-Bolaños Alfredo A   Konishi Kazuhisa K   Richards Thomas J TJ   Kaminski Naftali N   Pardo Annie A  

American journal of physiology. Lung cellular and molecular physiology 20130301 9


Lung fibrosis is the final result of a large number of disorders and is usually considered an irreversible process. However, some evidence suggests that fibrosis could eventually be reversible. In this study we aimed to document the time-related reversibility of bleomycin-induced lung fibrosis and to examine the gene expression profile associated with its initial progression and subsequent resolution. C57BL/6 mice were instilled with a single dose of bleomycin and euthanized at 1, 4, 8, 12, and  ...[more]

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