Dataset Information


Targeting the treponemal microbiome of digital dermatitis infections by deep coverage pyrosequencing

ABSTRACT: We examined 36 biopsies taken from digital dermatitis lesions of Holstein cows. The target was the V3 -V4 variable region of 16S rRNA using Treponema specific primers. We identified 20 different taxa of Treponema using this approach. Phylogenetic study of the Treponema taxa found in digital dermatitis lesions of Holstein cows.

ORGANISM(S): Bos taurus  

SUBMITTER: Mette Boye   Tim K Jensen  Kirstine Klitgaard  Antoni F Bretó 

PROVIDER: E-GEOD-42426 | ArrayExpress | 2013-06-25



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Targeting the treponemal microbiome of digital dermatitis infections by high-resolution phylogenetic analyses and comparison with fluorescent in situ hybridization.

Klitgaard Kirstine K   Foix Bretó Antoni A   Boye Mette M   Jensen Tim K TK  

Journal of clinical microbiology 20130508 7

Modern pyrosequencing technology allows for a more comprehensive approach than traditional Sanger sequencing for elucidating the etiology of bovine digital dermatitis. We sought to describe the composition and diversity of treponemes in digital dermatitis lesions by using deep sequencing of the V3 and V4 hypervariable regions of the 16S rRNA gene coupled with species-level taxonomic identification. Treponema-specific 16S rRNA gene PCRs and pyrosequencing were performed on biopsy specimens origin  ...[more]

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