Transcriptomics

Dataset Information

300

Transcriptome-profiling (RNA-seq) and Ribosome-profiling (Ribo-seq) in proliferation, quiescence, senescence and transformed states.


ABSTRACT: We applied in parallel RNA-Seq and Ribosome-profiling analyses to immortalized human primary BJ fibroblast cells under the following conditions: normal proliferation, quiescence (induced by serum depletion), senescence (induced by activation of the oncogenic RASG12V gene, and examined at early (5 days; pre-senescent state) and late (14 days; fully senescent state) time points), and neoplastic transformation (induced by RASG12V in the background of stable p53 and p16INK4A knockdowns and SV40 small-T expression. RNA-seq, using Illumina HiSeq 2000, was applied to BJ cells under 5 conditions: proliferation, quiescence, pre-senescence, full-senescence, and transfomed. Ribosome profiling, using Illumina HiSeq 2000, was applied to BJ cells under 5 conditions: proliferation, quiescence, pre-senescence, full-senescence, and transfomed.

ORGANISM(S): Homo sapiens  

SUBMITTER: Rani Elkon   Reuven Agami  Ran Elkon  Fabricio Loayza-Puch 

PROVIDER: E-GEOD-42509 | ArrayExpress | 2013-03-24

SECONDARY ACCESSION(S): SRP017378GSE42509PRJNA182756

REPOSITORIES: GEO, ArrayExpress, ENA

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